Chromatography is used to separate and analyse mixtures of soluble substances. The first steps in the method are given: place the sample on the baseline; allow the chromatogram to develop in solvent. What is the next stage in the process for detecting soluble colourless substances?
- Acalculate the $R_f$ values
- Bcompare the results with known substances
- Ccount the number of spots
- Duse a suitable locating agent