(a)[2]
Define enzyme.
(b(i))[1]
State the order of bases on the lower strand of the DNA molecule in step 1.
(b(ii))[1]
State the name of enzyme 1 shown in step 2 of Fig. 1.1.
(b(iii))[2]
Describe what enzyme 1 does to the DNA molecule in step 3.
(b(iv))[2]
Explain how enzyme 1 in Fig. 1.1 is specific for the exact sequence of DNA bases.
(c(i))[1]
State the type of organism that naturally contains plasmids.
(c(ii))[1]
State the name given to enzyme 2 in step 7 of Fig. 1.2.
(c(iii))[1]
State the name of the molecule produced in step 8.
(d)[3]
Sketch a graph to show how the activity of the enzymes used in genetic engineering would change at a range of temperatures from very cold to very hot. Label the axes with suitable titles. Do not include units or a numbered scale.