Biology 0610 · IGCSE · Genetic modification

Genetic modification — practice question

Several stages in the production of human insulin by genetic modification are given below. 1 insertion of the human insulin gene DNA into a bacterial plasmid that has been cut 2 bacteria containing recombinant plasmids multiply and produce human insulin 3 human insulin gene DNA is isolated using restriction enzymes 4 recombinant plasmids are inserted into bacteria What is the correct order of these processes in genetic modification?

  • A$1 \rightarrow 3 \rightarrow 2 \rightarrow 4$
  • B$1 \rightarrow 4 \rightarrow 3 \rightarrow 2$
  • C$3 \rightarrow 1 \rightarrow 4 \rightarrow 2$
  • D$3 \rightarrow 2 \rightarrow 1 \rightarrow 4$

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