Chemistry 9701 · AS & A Level · States of matter

States of matter — practice question

The isoelectric point is the pH at which an amino acid is present as a zwitterion. Glutamic acid has an isoelectric point of 3.2 and cysteine has an isoelectric point of 5.0. A mixture containing the dipeptide glu-cys and its two component amino acids, glutamic acid and cysteine, was separated by electrophoresis in a buffer at pH 5.2. The results are shown.
(a)[2]

Use the Data Booklet to help you draw the structure of the dipeptide glu-cys.

(b)[3]

Suggest the identities of the species responsible for spots E, F and G. Explain your reasons.

Worked solution & mark scheme

This 5-mark question has a full step-by-step worked solution and mark scheme. One marking point: The peptide linkage is correct

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