State the order of basicity for ethanamide, diethylamine and ethylamine in aqueous solution. Explain your answer.
The amino acid alanine, $\text{H}_2\text{NCH}(\text{CH}_3)\text{COOH}$, is able to act as a buffer.
Define a buffer solution.
Write two equations to show how an aqueous solution of alanine can behave as a buffer solution.
Another amino acid that acts as a buffer is glutamic acid.
Draw the skeletal formula of glutamic acid.
Draw the structure of the dipeptide, ala-glu, made from one molecule of alanine and one molecule of glutamic acid. The peptide bond that forms should be shown.
The isoelectric point of alanine is $6.0$ and of glutamic acid is $3.2$. A mixture containing the dipeptide, ala-glu, and its two component amino acids, alanine and glutamic acid, is examined by electrophoresis using a buffer at $\text{pH }6.0$. Draw and label three spots on Fig. 7.2 to show the predicted position of each of these three species after electrophoresis. Explain your answer.
Alanine, $\text{H}_2\text{NCH}(\text{CH}_3)\text{COOH}$, reacts with methanol under suitable conditions to produce the ester G. The proton ($^1\text{H}$) NMR spectrum of G dissolved in $\text{D}_2\text{O}$ is shown in Fig. 7.3.
Complete Table 7.2 for the proton ($^1\text{H}$) NMR spectrum of $G$.
The proton ($^1\text{H}$) NMR spectrum of $G$ dissolved in $\text{CDCl}_3$ is obtained. Describe the difference between this spectrum and the proton NMR spectrum in $\text{D}_2O$ shown in Fig. 7.3. Explain your answer.