Chemistry 9701 · AS & A Level · States of matter

States of matter — practice question

DNA fingerprinting has emerged as a highly significant method for examining samples taken from organisms that are alive now or were alive previously.
(a(i))[1]

After extraction and purification, what is the first stage in analysing a DNA sample?

(a(ii))[1]

What can be done to raise the amount of DNA available for analysis?

(a(iii))[2]

In electrophoresis, amino acids may move in different directions or not move at all, whereas DNA fragments always move in one direction. Explain these two observations.

(a(iv))[1]

DNA fingerprinting can also be useful in archaeology. Which of the following would not be suitable for analysis by DNA fingerprinting? Put a cross (x) in the appropriate box(es). - a strip of leather from an Egyptian tomb - skin from a mummified body - a shard of ancient pottery - wood from a Roman chariot

(b(i))[1]

X-ray crystallography can be used to help analyse the structure of macromolecules. What information does this technique provide about a particular macromolecule?

(b(ii))[1]

Which element will be seen most strongly in the X-ray crystallography of a biological polymer of general formula $\text{C}_v\text{H}_w\text{P}_x\text{N}_y\text{O}_z$? Explain your answer.

(c(i))[1]

What is meant by a partition coefficient?

(c(ii))[2]

A pesticide has a partition coefficient of $6.0$ between hexane and water. A solution contains $0.0042\text{ g}$ of the pesticide dissolved in $25\text{ cm}^3$ of water. The solution is shaken with $25\text{ cm}^3$ of hexane. Calculate the mass of pesticide that will be dissolved in the hexane layer at equilibrium.

Worked solution & mark scheme

This 10-mark question has a full step-by-step worked solution and mark scheme. One marking point: Cut DNA with restriction enzymes

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