Analytical techniques — Chemistry 9701 AS & A Level Question & Worked Solution

(a(i))[3]

Draw a labelled diagram of the apparatus used to separate a mixture by electrophoresis.

(a(ii))[2]

Explain the principles of the separation of amino acids by electrophoresis.

(b(i))[3]

Given buffers of pH 2.0, 7.0 and 12.0, suggest, with a reason, which buffer would be most suitable for the separation of Asp and Val.

(b(ii))[3]

Given buffers of pH 2.0, 7.0 and 12.0, suggest, with a reason, which buffer would be most suitable for the separation of Lys and Ser.

(b(iii))[3]

Given buffers of pH 2.0, 7.0 and 12.0, suggest, with a reason, which buffer would be most suitable for the separation of Tyr and Phe.

(c(i))[2]

Draw the structure of the dipeptide Gly-Ser, showing the peptide bond in full.

(c(ii))[2]

Use the Data Booklet to identify the bond in the molecule of Gly-Ser that is responsible for each of the peaks indicated on the infra-red spectrum.

Analytical techniques — practice question