Chemistry 9701 · AS & A Level · Analytical techniques

Analytical techniques — practice question

A protein can be analysed by first breaking it into its amino acids. These can then be separated by a process known as electrophoresis.
(a)[3]

The structures of glycine, lysine and glutamic acid at pH 7 are shown. On the chart below, draw and label three circles to show where each of these amino acids is likely to be after electrophoresis of a solution containing these amino acids in a buffer at pH 7.

(b(i))

State what the term partition coefficient means.

(b(ii))[3]

One concern about organic pollutants, such as pesticide residues, is that they can enter the food chain and accumulate in human breast milk. Explain how this can happen.

(c(i))

Identify the species responsible for each peak at $m/e = 156$, $158$ and $160$.

(c(ii))[4]

A large peak was present in the spectrum with a $m/e$ value below $20$. Suggest the $m/e$ value for the peak and the species that produced it.

Worked solution & mark scheme

This 10-mark question has a full step-by-step worked solution and mark scheme. One marking point: Glutamic acid positioned between positive electrode and start point

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