Chemistry 9701 · AS & A Level · Analytical techniques

Analytical techniques — practice question

A protein can be analysed by first breaking it into its amino acids. The amino acids can then be separated using a technique called electrophoresis.
(a)[3]

The structures of glycine, lysine and glutamic acid at pH 7 are shown. On the chart below, draw and label three circles to show the most likely position of each amino acid after electrophoresis of a solution containing these amino acids in a buffer at pH 7.

(b(i))

State what the term partition coefficient means.

(b(ii))[3]

One worry about organic pollutants, such as pesticide residues, is that they can move through the food chain and become concentrated in human breast milk. Explain how this occurs.

(c(i))

Name the species responsible for each of these peaks: $m/e = 156$, $m/e = 158$, and $m/e = 160$.

(c(ii))[4]

A prominent peak was present in the spectrum at an $m/e$ value below $20$. Suggest the $m/e$ value of the peak and the species that formed it.

Worked solution & mark scheme

This 10-mark question has a full step-by-step worked solution and mark scheme. One marking point: Glutamic acid between + and start point

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