Chemistry 9701 · AS & A Level · Analytical techniques

Analytical techniques — practice question

DNA fingerprinting has been among the most significant advances in biochemical analysis in recent years. It has allowed major progress in forensic science, medicine and archaeology.
(a)[3]

The table lists several stages involved in producing a genetic fingerprint. Put the stages into the right order in the empty column using the numbers $1$ to $6$.

(b(i))

A radioactive isotope is used in one of the stages above. Which isotope is used?

(b(ii))[2]

Why is this isotope selected?

(c)

The DNA fingerprints below were obtained from a family consisting of a mother, father and four children.

(c(i))

Are all of the children related to the mother? State the evidence for your answer.

(c(ii))[2]

Which child is least likely to be related to the father? State the evidence for your answer.

(d(i))

DNA fingerprinting has been used successfully in archaeological investigations. In the past, ancient writings were often produced on goatskins. Over many centuries these have frequently broken into fragments, making it almost impossible to rebuild the writings. Suggest how DNA fingerprinting could be used to identify which fragments came from a particular skin.

(d(ii))[3]

Apart from human remains and goatskins, state one other material that could be investigated using this technique.

Worked solution & mark scheme

This 10-mark question has a full step-by-step worked solution and mark scheme. One marking point: Accurate ordering of DNA fingerprinting stages

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