(a)[2]
Calculate the reaction’s initial rate.
(b(i))[2]
The investigation was carried out again to see how the pepsin activity was affected by a competitive inhibitor at the same temperature, $20^\circ\mathrm{C}$. Predict the outcome when the competitive inhibitor is used.
(b(ii))[2]
The investigation was repeated with no competitive inhibitor, but now at the higher temperature of $30\,^{\circ}\mathrm{C}$. Predict the outcome at $30\,^{\circ}\mathrm{C}$.
(c)[2]
The students broadened the investigation by using pepsin from another species of mammal. The experiments were done at $20\,^{\circ}\mathrm{C}$ and without a competitive inhibitor. Referring to Fig. 2.1, explain why calculating the initial rate of reaction is advantageous in each experiment.