Biology 9700 · AS & A Level · Structure of nucleic acids and replication of DNA

Structure of nucleic acids and replication of DNA — practice question

Cholera is an infectious disease brought about by a bacterium.
(a(i))[1]

Fig. 2.1 presents a transmission electron micrograph of this bacterium. Name the bacterium responsible for cholera.

(a(ii))[6]

The bacterium shown in Fig. 2.1 is a prokaryotic cell. Each description A to C refers to a cell structure found in both prokaryotic cells and plant cells. For each of A to C: name the structure described, and state one difference between a prokaryotic cell and a plant cell for that structure. A: the site where polypeptides are synthesised. B: the cell's genetic material. C: the rigid outer structure that keeps the cell in shape and prevents osmotic lysis.

(b)[2]

Cholera is one example of an infectious disease. Explain what the term infectious disease means.

(c)[2]

The symptoms of cholera are caused by choleragen, a toxin released by the bacterium. Choleragen is a protein composed of six polypeptides: one polypeptide called the A subunit, which contains an extended alpha helix, and five polypeptides that together form the B subunit. List the levels of protein structure present in choleragen.

(d)[3]

Outline the process by which choleragen enters the cell. You may use the space for annotated diagrams.

(e(i))[1]

Suggest why subunit B is chosen instead of subunit A for the vaccine.

(e(ii))[5]

Outline how this vaccine can protect against cholera.

Worked solution & mark scheme

This 20-mark question has a full step-by-step worked solution and mark scheme. One marking point: The bacterium is $Vibrio\ cholerae$.

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