Monoclonal antibodies are made for use in the diagnosis or treatment of disease. To collect the antibodies against an antigen, a mouse is injected with the antigen. Several stages in monoclonal antibody production are given below. 1 Plasma cells are fused with cancer cells to produce a hybridoma. 2 Hybridomas that release the needed antibody are found and cloned. 3 B-lymphocytes that recognise the antigen multiply and change into plasma cells. 4 Hybridomas divide by mitosis and secrete antibodies. 5 Plasma cells are taken from the mouse spleen. What is the order of the first four stages in the production of the monoclonal antibody?
- A3 5 1 2
- B3 5 1 4
- C5 1 2 4
- D5 1 4 2