Biology 9700 · AS & A Level · Proteins

Proteins — practice question

The table contains some details about the polypeptides that form haemoglobin. Researchers examined the part of the $\beta$-globin polypeptide chain that includes the amino acid cysteine at position $93$. They discovered that: • this region points outwards when no oxygen is bound to the haem group • this region points inwards when oxygen is bound to the haem group • substituting cysteine with another amino acid lowers the Bohr shift. What conclusion can be drawn from the information about cysteine in haemoglobin?

  • AMore than $1\%$ of the amino acids in one haemoglobin protein are cysteine.
  • BIn $\alpha$-globin, there is a cysteine closer to the end of the polypeptide chain with an unreacted carboxyl group than in $\beta$-globin.
  • CThe replacement of the cysteine at position $93$ in $\beta$-globin decreases the affinity of haemoglobin for oxygen at low pH.
  • DThe binding of oxygen to the haem group causes the region of $\beta$-globin containing cysteine at position $93$ to become more hydrophilic.

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