Biology 9700 · AS & A Level · Protein synthesis

Protein synthesis — practice question

Inside the mesophyll tissue of leaves, the products of photosynthesis may be used to build organic compounds, including the polysaccharide cellulose and certain amino acids. Nitrate ions absorbed by the roots and carried to the leaves can provide a nitrogen source for amino acid synthesis.
(a)[3]

Describe the features of the structure of a cellulose molecule.

(b(i))[1]

Researchers have found that adding nitrate to leaf tissue increases messenger RNA (mRNA) molecules of gene $NR$, which codes for nitrate reductase. State one advantage to leaf cells of an increase in mRNA molecules of gene $NR$ after nitrate is added.

(b(ii))[2]

One technique used to detect nitrite produced by the reduction of nitrate in leaf tissue involves: using an inhibitor to stop nitrite taking part in further reactions in the leaf tissue; placing the leaf tissue in a solution that contains a colourless test reagent. Nitrite from the leaf tissue moves into the surrounding solution, causing the solution to turn magenta (red-purple). Suggest why a colorimeter can improve this method for detecting nitrite.

(c(i))[4]

Outline and explain the sequence of events that allows amino acids to be carried from the apoplast into the cytoplasm of a companion cell.

(c(ii))[1]

Suggest why amino acid transporters are not required to move amino acids from the companion cell into a phloem sieve tube element.

(d)
(e)[4]

The nitrate uptake of excised maize roots was studied under a range of conditions. Table 4.1 gives the details and results for a control experiment and for four altered experiments, 1, 2, 3 and 4. The same nitrate-solution concentration was used in all experiments. All results were recorded after a fixed time period. The results for experiments 1, 2, 3 and 4 in Table 4.1 can be compared with the control experiment. Discuss how comparing each result with the control provides information about: - how nitrate ions are absorbed by the root cells - the factors that influence nitrate-ion uptake.

Worked solution & mark scheme

This 15-mark question has a full step-by-step worked solution and mark scheme. One marking point: Cellulose is composed of $\beta$-glucose monomers.

  • Full mark scheme, point by point
  • Step-by-step worked solution
  • Write your answer & get it marked instantly by AI