(a(i))[1]
Use Fig. 2.1 to find $V_{max}$ for trypsin.
(a(ii))[2]
Use Fig. 2.1 to calculate $K_m$ for trypsin. Show your working.
(b)[4]
Describe and explain the form of the curve shown in Fig. 2.1.
(c(i))[3]
Trypsin consists of one polypeptide chain of 223 amino acids. The active site of trypsin contains three amino acids that catalyse a hydrolysis reaction. In the primary structure of trypsin, these amino acids are found at positions 57 (histidine), 102 (aspartate) and 195 (serine). In the working enzyme, these three amino acids are close together in the active site. Explain how the structure of the protein makes this possible.
(c(ii))[1]
When trypsin acts on a substrate, a second substance is needed as a reactant. Name this other substance.