Enzymes consist of polymers made from amino acids. Complete Fig. 3.1 so that it shows the general structure of an amino acid.
Calculate the rate of reaction when the standard brown colour was reached after 2 minutes 30 seconds. The rate of reaction, $R$, was found using the formula $R = \frac{1}{\text{time to reach standard brown colour in minutes}}$.
Fig. 3.3 presents a graph of the investigation results. State how the data in Fig. 3.3 show that substrate was in excess at every concentration of catechol oxidase tested.
Explain the form of the curve shown in Fig. 3.4.
Use Fig. 3.4 to calculate the Michaelis-Menten constant ($K_m$) for the reaction involving catechol oxidase and catechol.
Methylcatechol is similar in shape to catechol. Catechol oxidase can also act on methylcatechol as a substrate. Under identical conditions, the $K_m$ value for the reaction with methylcatechol as the substrate was much lower than the $K_m$ value for the reaction with catechol as the substrate. State what these $K_m$ values reveal about the relationship between the enzyme and the two substrates.