Biology 9700 · AS & A Level · Principles of genetic technology
Principles of genetic technology — practice question
Green fluorescent protein (GFP) is a compact protein that produces bright green fluorescence under blue light. It was first isolated from the jellyfish, $Aequorea\ victoria$. The gene coding for GFP can be expressed in bacteria, such as $Escherichia\ coli$, and so it is commonly used as a marker to indicate successful uptake of a gene by the bacterium.
(a(i))[3]
Outline how a gene from a different species can be inserted into $E.\ coli$.
(a(ii))[3]
Explain how a marker gene, such as the gene for GFP, is used to show successful uptake of a gene for a wanted protein.
(b)[2]
Genes for enzymes that make fluorescent substances are often used as markers in gene technology. GFP is not an enzyme. Suggest one disadvantage of using the gene for GFP to create easily detectable fluorescence, rather than using a gene for an enzyme that makes a fluorescent substance. Explain your answer.
Worked solution & mark scheme
This 8-mark question has a full step-by-step worked solution and mark scheme. One marking point: “Target gene isolated” …