Name the method used to produce monoclonal antibodies.
Describe what the term monoclonal antibody means.
Describe, in detail, the molecular structure of one of the antibodies made in step 6. You may want to use an annotated diagram in your answer.
Suggest why cells with this gene mutation are unable to grow in a culture medium containing HAT.
Explain why the mutant myeloma cells are able to grow in a culture medium containing HAT once they have been fused with mouse spleen cells (steps 3 and 4).
Suggest why growing the fused cells in a culture medium containing HAT (step 4) is an important part of the procedure shown in Fig. 3.1.
The mutant myeloma cells used in step 2 are myeloma cells with a gene mutation that stops them from growing in a culture medium containing hypoxanthine, aminopterin and thymidine (HAT).
Suggest advantages of using monoclonal antibodies in pregnancy testing.