Name the disease that is treated with recombinant human factor VIII.
Before recombinant human factor VIII became available, factor VIII taken from donated blood was used to treat this disease. Give two advantages of using recombinant human factor VIII instead of factor VIII from donated blood to treat this disease.
The gene that codes for human factor VIII can be made from messenger RNA purified from human liver cells. Name the enzyme that uses messenger RNA as a template to make complementary DNA.
Outline two sources, other than messenger RNA, from which genes may be obtained for genetic engineering.
The gene that codes for human factor VIII can be transferred into mammalian cells in tissue culture. Explain why a promoter also has to be transferred into the mammalian cells so that human factor VIII can be synthesised.