Biology 9700 · AS & A Level · Movement into and out of cells

Movement into and out of cells — practice question

An investigation was done to find out how different treatments affect the permeability of the cell surface membranes and tonoplasts in beetroot cells. The vacuoles of beetroot cells contain a red pigment. This pigment cannot leave the cells because it is unable to diffuse through the tonoplasts or the cell surface membranes. $1\text{ cm}^3$ cubes were taken from beetroot tissue and rinsed under running water for 20 minutes to remove any pigment released from damaged cells. The cubes were then put into test-tubes containing different contents and left for five minutes before being observed. Which row gives a correct explanation for the observation recorded for one of the treatments?

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