Biology 9700 · AS & A Level · Mode of action of enzymes

Mode of action of enzymes — practice question

When exposed to ultraviolet light, some molecules show fluorescence. If the structure or shape of these molecules changes, the fluorescence intensity can also change. The enzyme tryptophan synthase shows bluish-green fluorescence because of a phosphate group linked with the active site. The enzyme catalyses the reaction shown. $\text{serine} + \text{indole} \rightarrow \text{tryptophan}$ When serine is added to the enzyme, the fluorescence intensity increases, but if indole is then added as well, the bluish-green fluorescence falls in intensity. What conclusion may be drawn from these observations?

  • A1, 2 and 3
  • B1 and 2 only
  • C1 and 3 only
  • D2 and 3 only

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