Biology 9700 · AS & A Level · Mode of action of enzymes

Mode of action of enzymes — practice question

The enzyme $\beta$-galactosidase is able to catalyse the hydrolysis of four substrates that have similar structures. Each substrate produces a different $K_m$ value. Which substrate does $\beta$-galactosidase have the greatest affinity for?

  • Asubstrate 1, $K_m = 4 \times 10^{-3}\ \text{mol dm}^{-3}$
  • Bsubstrate 2, $K_m = 1 \times 10^{-3}\ \text{mol dm}^{-3}$
  • Csubstrate 3, $K_m = 2 \times 10^{-4}\ \text{mol dm}^{-3}$
  • Dsubstrate 4, $K_m = 1 \times 10^{-4}\ \text{mol dm}^{-3}$

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