Biology 9700 · AS & A Level · Gene control

Gene control — practice question

The $\textit{lac}$ operon is a DNA segment found in the genome of $\textit{Escherichia coli}$. The structural genes in the $\textit{lac}$ operon are fully expressed only when the bacteria experience high lactose concentrations. Fig. 6.1 is a diagram of the $\textit{lac}$ operon and an adjacent section of the $E.\ coli$ genome.
(a(i))[2]

Fig. 6.1 illustrates that the $\textit{lac}$ operon includes structural genes and regulatory sequences. Use Fig. 6.1 to pick out two structural genes. Complete Table 6.1 by naming each structural gene and its product.

(a(ii))[1]

Gene I is transcribed continuously so that its protein is made. This is constitutive expression. Explain why some genes show constitutive expression.

(a(iii))[2]

Describe how the product of gene I affects the functioning of the $\textit{lac}$ operon.

(b(i))[2]

If $E.\ coli$ is placed in a nutrient medium that contains lactose, some new enzymes are made. These are called inducible enzymes. Explain what is meant by an inducible enzyme.

(b(ii))[1]

The structural genes of the $\textit{lac}$ operon are not expressed when lactose is absent. Suggest one reason why this benefits $E.\ coli$.

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This 8-mark question has a full step-by-step worked solution and mark scheme. One marking point: $lacZ$ encodes $\beta$-galactosidase

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