Biology 9700 · AS & A Level · Factors that affect enzyme action

Factors that affect enzyme action — practice question

Pyrophosphatase enzymes bring about a hydrolysis reaction. $\text{pyrophosphate ions} \xrightarrow{\text{pyrophosphatase}} \text{phosphate ions}$ During experiment 1, a scientist investigated the rate of this reaction using a colorimeter. The absorbance of the solution was recorded at fixed intervals until all the pyrophosphate ions had been changed into phosphate ions. For experiment 2, the scientist carried out the procedure again with a greater concentration of pyrophosphatase. All other variables were kept constant. Which graph shows the effect of increasing the concentration of pyrophosphatase?

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