An experiment was done to examine how temperature affects an enzyme-catalysed reaction. At first, the enzyme and its substrate were kept in separate test-tubes and brought up to the necessary temperature. They were then combined and transferred into four tubes A, B, C and D. Each tube was incubated for the stated time and temperature. After that, the mass of product produced was measured. In which tube did the reaction occur at the greatest rate?
- A30 s, 25 ^{\circ}C, 2.5 µg
- B30 s, 45 ^{\circ}C, 5.0 µg
- C600 s, 25 ^{\circ}C, 32.0 µg
- D600 s, 45 ^{\circ}C, 10.0 µg