Biology 9700 · AS & A Level · Factors that affect enzyme action

Factors that affect enzyme action — practice question

An investigation was conducted in which the enzyme lipase was used to hydrolyse a triglyceride. pH was measured at set intervals throughout the reaction. The findings are shown in the table. At 14 minutes, unreacted triglyceride was still present. What is the explanation for the results after 10 minutes?

  • AThe end-products acted as competitive inhibitors.
  • BThe end-products acted as non-competitive inhibitors.
  • CThe enzyme reaction had reached $V_{\text{max}}$.
  • DThe tertiary structure of the enzyme had been lost.

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