The graph illustrates how an increase in substrate concentration affects the rate of an enzyme-catalysed reaction. Line P shows the outcome when the enzyme is used at its optimum pH and optimum temperature, with no inhibitor present. Line Q shows the outcome when the reaction conditions are altered. Which descriptions of changes to the reaction conditions could produce line Q if all the other conditions remained unchanged? 1. Introduce an inhibitor that binds to a site other than the active site. 2. Introduce an inhibitor with a shape similar to the substrate. 3. Introduce an inhibitor that prevents the active site of the enzyme from working. 4. Perform the reaction at a higher temperature.
- A1, 3 and 4
- B1 and 4 only
- C2, 3 and 4
- D2 and 3 only