In an investigation, an equal concentration of the enzyme phosphorylase was mixed with different concentrations of glucose phosphate and left at $30^\circ\text{C}$. At intervals of $1$ minute, a single drop of the reaction mixture was taken and placed onto a drop of iodine solution on a white tile. The diagram presents the outcomes of this investigation. What accounts for the pattern seen in the results of this investigation?
- APhosphorylase catalyses a reaction converting glucose phosphate to starch.
- BThe maximum rate of reaction is reached at $20\,\text{mg dm}^{-3}$ of glucose phosphate.
- CSubstrate concentration is limiting at concentrations of glucose phosphate $25\,\text{mg dm}^{-3}$ or less.
- DEnzyme concentration is limiting at concentrations of glucose phosphate $25\,\text{mg dm}^{-3}$ or less.