(a)[3]
Hexokinase binds glucose by the induced fit mechanism. Describe how an enzyme-substrate complex forms by this mechanism.
(b)[2]
Suggest how enzymes that use the induced fit mechanism can be less affected by competitive inhibitors than those that use the lock and key mechanism.
(c)[3]
When a solution of the enzyme hexokinase is heated to $45^\circ\text{C}$ for $10$ minutes, the amount of product formed falls by $50\%$ compared with a sample maintained between $30^\circ\text{C}$ and $40^\circ\text{C}$. Explain this result.
(d(i))[2]
State two differences between active transport and facilitated diffusion.
(d(ii))[2]
Suggest why glucose-6-phosphate cannot move out of cells.