State the term used for an enzyme that is released outside the cell and acts there.
Fig. 2.1 shows one of the triglycerides present in olive oil.
On Fig. 2.1, draw an arrow to show where lipase digestion breaks the bond between the glycerol residue and the stearic acid residue.
State the type of reaction that produces stearic acid from this triglyceride and name the type of bond that is broken. Type of reaction Type of bond
The activity of the bacterial lipase was measured at $37\,^{\circ}\text{C}$ using different concentrations of olive oil as the substrate. The results were used to obtain a Michaelis-Menten constant $(K_m)$ of $91.76\,\text{mmol dm}^{-3}$. Explain what is meant by a $K_m$ of $91.76\,\text{mmol dm}^{-3}$.
Fig. 2.2 presents the findings of an investigation into how temperature affects the activity of bacterial lipase.
Describe and explain the pattern shown in Fig. 2.2.
An investigation using human pancreatic lipase over the same temperature range would give results that differ from those in Fig. 2.2. Predict how the trend would differ from the one shown in Fig. 2.2.
Describe and explain the results presented in Fig. 2.2.
An investigation using human pancreatic lipase across the same temperature range would produce results unlike those in Fig. 2.2. Predict how the trend would differ from the one shown in Fig. 2.2.