(a)[2]
Finish Fig. 2.1 so it shows triglyceride hydrolysis by lipase: triglyceride + .................... $\xrightarrow{\text{lipase}}$ ....................
(b)[3]
Outline a biological test that could be used to show that triglyceride is present in a liquid mixture, and describe what a positive result looks like.
(c)[4]
With reference to Fig. 2.2, describe how pH affects bacterial lipase activity.
(d(i))[1]
Suggest one reason why the optimum pH of the free enzyme in solution differs from that of the immobilised enzyme.
(d(ii))[1]
Suggest one advantage of immobilising the extracted lipase for commercial use.